Inhibition of adenylosuccinate synthetase and adenylosuccinate lyase from Ehrlich ascites-tumour cells by 6-thioinosine 5'-phosphate.
نویسندگان
چکیده
Szent-Gyorgyi, A. G. (1953). Arch. Biochem. Biophys. 42, 305. Taylor, J. F., Green, A. A. & Cori, G. T. (1948). J. biol. Chem. 173, 591. Tulpule, P. G. & Kshirsagar, S. G. (1958). Indian J. med. Re8. 46, 452. Tulpule, P. G. & Patwardhan, V. N. (1952). Arch. Biochem. Biophy8. 39, 450. Waterlow, J. C. & Mendes, C. B. (1957). Nature, Lond., 180, 1361. Waterlow, J. C. & Weisz, T. (1956). J. clin. Inve.st. 35, 346. Wesson, L. G. (1932). Science, 75, 339. Widdowson, E. M. & McCance, R. A. (1957). Brit. J. Nutr. 11, 198. Zimmerman, M. J. & Weinstein, H. G. (1956). J. Lab. clin. Med. 48, 607.
منابع مشابه
Structural Modifications of Hadacidin and Their Effects on the Activity of Adenylosuccinate Synthetase.
Previous work from this laboratory demonstrated that hadacidin (N-formylhydroxyaminoacetate) inhibited the biosynthesis & luwo of adenylic acid in Ehrlich ascites tumor and rat liver cells. This effect, which was shown to be due to the inhibition of adenylosuccinate synthetase (IMP : L-aspartate ligase (1))) was competitively reversed by L-aspartate (2, 3). The structural similarity between asp...
متن کاملPurification of adenylosuccinate synthetase from rabbit skeletal muscle.
Adenylosuccinate synthetase has been purified more than 250-fold from extracts of rabbit muscle acetone powder by heating to 60”, ammonium sulfate fractionation, phosphocellulose and hydroxylapatite chromatography, and Sephadex G-150 gel filtration. Crystals have been obtained by dialysis against 60 to 67% ammonium sulfate. Enzyme activity is stable for about 2 weeks in phosphate buffer contain...
متن کاملInhibition of de novo purine biosynthesis and interconversion by 6-methylpurine in Escherichia coli.
The inhibition of Escherichia coli strain B and strain W-11 by 6-methylpurine depended on the formation of 6-methylpurine ribonucleotide by the action of adenine phosphoribosyltransferase (AMP: pyrophosphate phosphoribosyltransferase, EC 2.4.2.7). 6-Methylpurine ribonucleotide inhibited the de novo synthesis of purines, presumably via pseudofeedback inhibition of phosphoribosylpyrophosphate ami...
متن کاملInhibition by 6-mercaptopurine of Purine Phosphoribosyltransferases from Ehrlich Ascites-tumour Cells That Are Resistant to the Drug.
1. A strain of Ehrlich ascites-tumour cells that showed little inhibition of growth in the presence of 6-mercaptopurine accumulated less than 5% as much 6-thioinosine 5'-phosphate in vivo, in the presence of 6-mercaptopurine, as did the sensitive strain from which it was derived. 2. Specific activities of the phosphoribosyltransferases that convert adenine, guanine, hypoxanthine and 6-mercaptop...
متن کاملDisruption of the purine nucleotide cycle by inhibition of adenylosuccinate lyase produces skeletal muscle dysfunction.
Controversy exists as to whether the purine nucleotide cycle is important in normal skeletal muscle function. Patients with disruption of the cycle from a deficiency of AMP deaminase exhibit variable degrees of muscle dysfunction. An animal model was used to examine the effect of inhibition of the purine nucleotide cycle on muscle function. When the compound 5-amino-4-imidazolecarboxamide ribos...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Biochemical journal
دوره 92 2 شماره
صفحات -
تاریخ انتشار 1964